Switch #:
SWTI000679
Switch type:
Binary
Switch subtype:
Physicochemical compatibility

Description:
Phosphorylation of S458 in the PCNA-binding motif of DNA polymerase delta subunit 3 (POLD3) by cAMP subfamily reduces the affinity of binding to the Proliferating cell nuclear antigen (PCNA) and decreases the processivity of the polymerase complex.

Participants:
(1) DNA polymerase delta subunit 3 (POLD3)
(2) Proliferating cell nuclear antigen (PCNA)

Interactions
Interaction #1 POLD3 - PCNA

Interfaces
(1) LIG_PCNA_PIPBox_1 motif (456QVSITGFFQR465) in DNA polymerase delta subunit 3 (POLD3)
(2) Proliferating cell nuclear antigen, C-terminal domain (127-254) in Proliferating cell nuclear antigen (PCNA)

Interaction Regulation
PTM-dependent Inhibition (Phosphorylation of S458 on DNA polymerase delta subunit 3 (POLD3)) of the DNA polymerase delta subunit 3 (POLD3) LIG_PCNA_PIPBox_1 motif - Proliferating cell nuclear antigen (PCNA) Proliferating cell nuclear antigen, C-terminal domain interaction

Regulatory Enzymes for switch
Modifying enzymes for residue: S458: cAMP subfamily

Inferred Regulatory Enzymes for switch
Putative modifying enzymes for residue: S458 : cAMP-dependent protein kinase catalytic subunit alpha (PRKACA).

Context
Switch localisation
Curated: nucleus
ELM curated: nucleus
Inferred: nucleoplasm

Switch cell cycle phase
Curated: mitotic S phase

Switch pathways
Inferred: Mismatch repair, DNA replication, Nucleotide excision repair, Base excision repair
References

(1) Phosphorylation of the p68 subunit of Pol δ acts as a molecular switch to regulate its interaction with PCNA.
Rahmeh et al. Biochemistry (2012)




Loading visualisation

↑