Switches.ELM

Switch #:

SWTI000125

Switch type:
Binary

Switch subtype:
Physicochemical compatibility

Description:
Phosphorylation of T603 in the FHA-binding motif of DNA repair protein RAD9 (RAD9) induces binding to the Serine/threonine-protein kinase RAD53 (RAD53) protein.

Participants:
(1) DNA repair protein RAD9 (RAD9)
(2) Serine/threonine-protein kinase RAD53 (RAD53)

Interactions

Interaction #1 RAD9 - RAD53

Interfaces
(1) LIG_FHA_1 motif (₆₀₁ELTQELP₆₀₇) in DNA repair protein RAD9 (RAD9)
(2) FHA domain_(601-681) in Serine/threonine-protein kinase RAD53 (RAD53)

Interaction Regulation
PTM-dependent Induction (Phosphorylation of T₆₀₃ on DNA repair protein RAD9 (RAD9)) of the DNA repair protein RAD9 (RAD9) LIG_FHA_1 motif - Serine/threonine-protein kinase RAD53 (RAD53) FHA domain interaction

Additional Information
Affinity : 12.9 µM
Structural information: 1K2N

Context

References

(1) Solution structure of the yeast Rad53 FHA2 complexed with a phosphothreonine peptide pTXXL: comparison with the structures of FHA2-pYXL and FHA1-pTXXD complexes.
Byeon et al. J. Mol. Biol. (2001)