Switch #: SWTI000303

Switch type: Pre-assembly

Switch subtype: Composite binding site formation

Description:
Binding of von Willebrand factor (VWF) to integrin receptors depends on pre-assembly of Integrin alpha-V (ITGAV)-Integrin beta-3 (ITGB3) heterodimers, since association of the integrin alpha and beta subunits results in the formation of a composite binding site for the RGD motif in the ligand.

Participants:
(1) Integrin alpha-V (ITGAV)
(2) Integrin beta-3 (ITGB3)
(3) von Willebrand factor (VWF)

This is a sequential switch. The interactions in this switch take place in a defined order. The order is defined by numbers to the left of each interaction. More than one interaction can occur in each step, these interactions occur independently but are necessary for future step

1Interaction #1 ITGAV - ITGB3

Interfaces
(1) FG-GAP repeat in Integrin alpha-V (ITGAV)
(2) Integrin, beta chain _(38-461) in Integrin beta-3 (ITGB3)

2Interaction #2 VWF - Interaction #1 ITGAV - ITGB3

Requires Interaction #1 ITGAV - ITGB3

Interfaces
(3) LIG_RGD motif (₂₅₀₇RGD₂₅₀₉) in von Willebrand factor (VWF)
(4) Interaction #1 ITGAV - ITGB3

(1) Biosynthetic and functional properties of an Arg-Gly-Asp-directed receptor involved in human melanoma cell attachment to vitronectin, fibrinogen, and von Willebrand factor.
Cheresh et al. J. Biol. Chem. (1987)