Switch #:
Switch type:
Switch subtype:
Composite binding site formation

Binding of Protein TIFY 10A (TIFY10A) to Coronatine-insensitive protein 1 (COI1), an F-box substrate recognition subunit of the SCF E3 ubiquitin ligase, depends on binding of the plant hormone jasmonate to COI1, as this pre-assembled complex provides a composite binding site for the degron motif in TIFY10A. Subsequent ubiquitylation of TIFY10A by the SCF targets this transcriptional repressor for proteasomal degradation, a pre-requisite for the transcriptional response to jasmonate.

(2) Coronatine-insensitive protein 1 (COI1)
(3) Protein TIFY 10A (TIFY10A)

This is a sequential switch. The interactions in this switch take place in a defined order. The order is defined by numbers to the left of each interaction. More than one interaction can occur in each step, these interactions occur independently but are necessary for future step
1Interaction #1 TIFY10A - COI1

(1) N-[(3R,7S)-(+)-7-iso-Jasmonoyl]-(S)-isoleucine in
(2) Coronatine-insensitive protein 1 (COI1)
2Interaction #2 TIFY10A - Interaction #1 - - COI1

Requires Interaction #1 - - COI1

(3) DEG_SCF_COI1_1 motif (203IARRASLHRFLEKRKDRV220) in Protein TIFY 10A (TIFY10A)
(4) Interaction #1 - - COI1

(1) Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor.
Sheard et al. Nature (2010)

(2) A critical role of two positively charged amino acids in the Jas motif of Arabidopsis JAZ proteins in mediating coronatine- and jasmonoyl isoleucine-dependent interactions with the COI1 F-box protein.
Melotto et al. Plant J. (2008)

(3) JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling.
Thines et al. Nature (2007)

Loading visualisation