Switch #: SWTI000688

Switch type: Specificity

Switch subtype: Domain hiding

Description:
The KEN-box motif of APC/C-CDH1 modulator 1 (ACM1) binds to the substrate recruitment site of APC/C activator protein CDH1 (CDH1), the substrate recognition subunit of the Anaphase Promoting Complex/Cyclosome (APC/C), and thereby blocks recruitment, and subsequent targeting for proteasomal degradation, of the Cdh1 substrate Kinesin-like protein CIN8 (CIN8). Degradation of Kinesin-like protein CIN8 (CIN8) is required for mitotic exit and maintenance of the G1 phase of the cell cycle and is allowed by Cdc20-dependent degradation of APC/C-CDH1 modulator 1 (ACM1) in anaphase.

Participants:
(1) APC/C-CDH1 modulator 1 (ACM1)
(2) APC/C activator protein CDH1 (CDH1)
(3) Kinesin-like protein CIN8 (CIN8)

(1) Mechanisms of pseudosubstrate inhibition of the anaphase promoting complex by Acm1.
Burton et al. EMBO J. (2011)

(2) Unique D box and KEN box sequences limit ubiquitination of Acm1 and promote pseudosubstrate inhibition of the anaphase-promoting complex.
Choi et al. J. Biol. Chem. (2008)