Switch #:
SWTI000686
Switch type:
Specificity
Switch subtype:
Domain hiding

Description:
Binding of the second KEN-box motif of Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), a subunit of the Spindle Assembly Checkpoint (SAC), to the substrate recruitment site of Cell division cycle protein 20 homolog (CDC20), the substrate recognition subunit of the Anaphase Promoting Complex/Cyclosome (APC/C), blocks binding of the Cdc20 substrate Securin (PTTG1). As a result, Securin (PTTG1) is not targeted for proteasomal degradation until metaphase, when the SAC is inhibited. Destruction of Securin (PTTG1) is required for progression to the anaphase of the cell cycle.

Participants:
(1) Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B)
(2) Cell division cycle protein 20 homolog (CDC20)
(3) Securin (PTTG1)

Interactions
Interaction #1 BUB1B - CDC20

Interfaces
(1) DEG_APCC_KENBOX_2 motif (303AKENE307) in Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B)
(2) WD domain, G-beta repeat (-) in Cell division cycle protein 20 homolog (CDC20)
Interaction #2 PTTG1 - CDC20

Interfaces
(3) DEG_APCC_DBOX_1 motif (60TRKALGTVN68) in Securin (PTTG1)
(4) WD domain, G-beta repeat (-) in Cell division cycle protein 20 homolog (CDC20)

Interaction Regulation
Effector binding Abrogation (Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B)) of the Securin (PTTG1) DEG_APCC_DBOX_1 motif - Cell division cycle protein 20 homolog (CDC20) WD domain, G-beta repeat interaction

Context
Switch localisation
Curated: cytosol
ELM curated: cytosol, nucleus
Inferred: cytosol, perinuclear region of cytoplasm


Switch pathways
Inferred: Oocyte meiosis
References

(1) BubR1 blocks substrate recruitment to the APC/C in a KEN-box-dependent manner.
Lara-Gonzalez et al. J. Cell. Sci. (2011)




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