Description: Binding of the second KEN-box motif of Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), a subunit of the Spindle Assembly Checkpoint (SAC), to the substrate recruitment site of Cell division cycle protein 20 homolog (CDC20), the substrate recognition subunit of the Anaphase Promoting Complex/Cyclosome (APC/C), blocks binding of the Cdc20 substrate G2/mitotic-specific cyclin-B1 (CCNB1). As a result, G2/mitotic-specific cyclin-B1 (CCNB1) is not targeted for proteasomal degradation until metaphase, when the SAC is inhibited. Destruction of G2/mitotic-specific cyclin-B1 (CCNB1) is required for progression to the anaphase of the cell cycle.
Participants: (1) Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B) (2) Cell division cycle protein 20 homolog (CDC20) (3) G2/mitotic-specific cyclin-B1 (CCNB1)
(1) BubR1 blocks substrate recruitment to the APC/C in a KEN-box-dependent manner.Lara-Gonzalez et al. J. Cell. Sci. (2011)
