Switch #: SWTI000672

Switch type: Binary

Switch subtype: Pre-translational

Description:
Alternative splicing removes the Kinesin Light Chain-binding motif of Pleckstrin homology domain-containing family M member 2 (PLEKHM2), abrogating binding to Kinesin light chain 2 (KLC2). Pleckstrin homology domain-containing family M member 2 (PLEKHM2) contains kinesin light chain (KLC) binding WD motifs (second motif at 205-210), and these are required for kinesin-1 recruitment and the peripheral movement of lysosomes. ADP-ribosylation factor-like protein 8B (ARL8B) and PLEKHM2 act together to recruit kinesin-1 to lysosomes and hence direct their movement toward microtubule plus ends. A splice variant of PLEKHM2 that lacks a light chain binding motif does not stimulate movement, suggesting fine-tuning by alternative splicing.

Participants:
(1) Pleckstrin homology domain-containing family M member 2 (PLEKHM2)
(2) Kinesin light chain 2 (KLC2)

(1) Arl8 and SKIP act together to link lysosomes to kinesin-1.
Rosa-Ferreira et al. Dev. Cell (2011)