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Alternative splicing removes the EH-binding motif of Synaptojanin-1 (SYNJ1), abrogating binding to Epidermal growth factor receptor substrate 15 (EPS15). Isoform Synaptojanin-170 of Synaptojanin-1 (SYNJ1) is associated with clathrin-mediated endocytosis as a negative regulator of coat-membrane interaction. This isoform is virtually absent from mature neuronal synapses, where clathrin-mediated endocytosis plays a critical role and where Isoform Synaptojanin-145 of Synaptojanin-1 (SYNJ1) is by far the major synaptojanin isoform. Isoform Synaptojanin-145 of Synaptojanin-1 (SYNJ1) may be recruited early to clathrin-coated pits of synapses, either indirectly by adaptors that bind clathrin and AP-2, such as Amphiphysin (AMPH), or by interactions of endophilin family dimers with synaptic vesicle cargo, such as the vesicular glutamate receptor transport.

(1) Synaptojanin-1 (SYNJ1)
(2) Epidermal growth factor receptor substrate 15 (EPS15)

Interaction #1 SYNJ1 - EPS15

(1) LIG_EH_1 motif (1403TNPFS1407) in Synaptojanin-1 (SYNJ1)
(2) Epidermal growth factor receptor substrate 15 (EPS15)

Interaction Regulation
Alternative splicing Abrogation of the Synaptojanin-1 (SYNJ1) LIG_EH_1 motif - Epidermal growth factor receptor substrate 15 (EPS15) interaction


(1) Direct interaction of the 170 kDa isoform of synaptojanin 1 with clathrin and with the clathrin adaptor AP-2.
Haffner et al. Curr. Biol. (2000)

(2) Two synaptojanin 1 isoforms are recruited to clathrin-coated pits at different stages.
Perera et al. Proc. Natl. Acad. Sci. U.S.A. (2006)

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