Switch #:
SWTI000486
Switch type:
Binary
Switch subtype:
Pre-translational

Description:
Alternative splicing removes the PDZ-binding motif of Plasma membrane calcium-transporting ATPase 4 (ATP2B4), abrogating binding to Disks large homolog 1 (DLG1). A much lower affinity was recorded for the third PDZ domain of DLG1 (in in the micromolar range (KD = 1.2 microM) compared to nanomolar affinity (KD = 1.6 nM)). PMCA4b and DLG1 are co-expressed in kidney and intestinal epithelial cells as well as in several areas of the brain. Should be noted that the 'b' isoforms of Plasma membrane calcium-transporting ATPase 2 (ATP2B2) bind much more weakly to all PDZ domains of DLG1

Participants:
(1) Plasma membrane calcium-transporting ATPase 4 (ATP2B4)
(2) Disks large homolog 1 (DLG1)

Interactions
Interaction #1 ATP2B4 - DLG1

Interfaces
(1) LIG_PDZ_Class_1 motif (1236SLETSV1241) in Plasma membrane calcium-transporting ATPase 4 (ATP2B4)
(2) PDZ domain (Also known as DHR or GLGF) (224-310) in Disks large homolog 1 (DLG1)

Interaction Regulation
Alternative splicing Abrogation of the Plasma membrane calcium-transporting ATPase 4 (ATP2B4) LIG_PDZ_Class_1 motif - Disks large homolog 1 (DLG1) PDZ domain (Also known as DHR or GLGF) interaction

Additional Information
Affinity : hDLG PDZ1/2: 0.0016 µM, hDLG PDZ3: 1.2 µM
Context
References

(1) Plasma membrane Ca2+ ATPase isoform 4b binds to membrane-associated guanylate kinase (MAGUK) proteins via their PDZ (PSD-95/Dlg/ZO-1) domains.
Kim et al. J. Biol. Chem. (1998)




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