Switch #:
SWTI000353
Switch type:
Avidity-sensing

Description:
Phosphorylation of two 14-3-3-binding motifs in Protein kinase C epsilon type (PRKCE) induces high-avidity binding to dimeric 14-3-3 protein zeta/delta (YWHAZ).

Participants:
(1) 14-3-3 protein zeta/delta (YWHAZ)
(2) Protein kinase C epsilon type (PRKCE)

Interactions
This is a sequential switch. The interactions in this switch take place in a defined order. The order is defined by numbers to the left of each interaction. More than one interaction can occur in each step, these interactions occur independently but are necessary for future step
1Interaction #1 YWHAZ - YWHAZ

Interfaces
(1) 14-3-3 protein in 14-3-3 protein zeta/delta (YWHAZ)
(2) 14-3-3 protein (3-236) in 14-3-3 protein zeta/delta (YWHAZ)
Additional Information
Structural information: 2WH0
2Interaction #2 PRKCE - Interaction #1 YWHAZ - YWHAZ

Requires Interaction #1 YWHAZ - YWHAZ

Interfaces
(3) LIG_14-3-3_3 motif (343RSKSAP348) in Protein kinase C epsilon type (PRKCE)
(4) Interaction #1 YWHAZ - YWHAZ

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S346 on Protein kinase C epsilon type (PRKCE)) of the Protein kinase C epsilon type (PRKCE) LIG_14-3-3_3 motif - 14-3-3 protein interaction

Additional Information
Affinity : pS346-S368: 9.8 µM, S346-pS368: >100 µM, pS346-pS368: 0.059 µM
Structural information: 2WH0
2Interaction #3 PRKCE - Interaction #1 YWHAZ - YWHAZ

Requires Interaction #1 YWHAZ - YWHAZ

Interfaces
(5) LIG_14-3-3_3 motif (365KALSFD370) in Protein kinase C epsilon type (PRKCE)
(6) Interaction #1 YWHAZ - YWHAZ

Interaction Regulation
PTM-dependent Induction (Phosphorylation of S368 on Protein kinase C epsilon type (PRKCE)) of the Protein kinase C epsilon type (PRKCE) LIG_14-3-3_3 motif - 14-3-3 protein interaction


Additional Information
Affinity : pS346-S368: 9.8 µM, S346-pS368: >100 µM, pS346-pS368: 0.059 µM
Structural information: 2WH0
Context
References

(1) Recognition of an intra-chain tandem 14-3-3 binding site within PKCepsilon.
Kostelecky et al. EMBO Rep. (2009)




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