Description: Phosphorylation of Isoform Nedd4-2a of E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) by Serine/threonine-protein kinase Sgk1 (SGK1) induces binding to 14-3-3 protein eta (YWHAH). This inhibits (whether allosterically or sterically is not known) interactions of NEDD4L via its WW domains with the PY motif in Amiloride-sensitive sodium channel subunit gamma (SCNN1G) (ENaC). As a result, ENaC does not get degraded and ENaC-mediated Na+ currents increase.
Participants: (1) Isoform Nedd4-2a of E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (2) 14-3-3 protein eta (YWHAH) (3) Amiloride-sensitive sodium channel subunit gamma (SCNN1G)
(1) 14-3-3 Mediates phosphorylation-dependent inhibition of the interaction between the ubiquitin E3 ligase Nedd4-2 and epithelial Na+ channels.Nagaki et al. Biochemistry (2006)
