Switch #:
SWTI000209
Switch type:
Binary
Switch subtype:
Physicochemical compatibility

Description:
Phosphorylation of T1269 in the PDZ-binding motif of Breakpoint cluster region protein (BCR) inhibits its interaction with the Afadin (MLLT4) protein.

Participants:
(1) Breakpoint cluster region protein (BCR)
(2) Afadin (MLLT4)

Interactions
Interaction #1 BCR - MLLT4

Interfaces
(1) LIG_PDZ_Class_1 motif (1266LFSTEV1271) in Breakpoint cluster region protein (BCR)
(2) PDZ domain (Also known as DHR or GLGF) (1008-1090) in Afadin (MLLT4)

Interaction Regulation
PTM-dependent Abrogation (Phosphorylation of T1269 on Breakpoint cluster region protein (BCR)) of the Breakpoint cluster region protein (BCR) LIG_PDZ_Class_1 motif - Afadin (MLLT4) PDZ domain (Also known as DHR or GLGF) interaction

Additional Information
Affinity : BCR (Wild type): 85.9 µM, BCR (T1269D mutant): >1000 µM
Structural information: 2AIN
Context
Switch localisation
ELM curated: cytosol, internal side of plasma membrane
Inferred: cytosol
References

(1) Solution structure and backbone dynamics of the AF-6 PDZ domain/Bcr peptide complex.
Chen et al. Protein Sci. (2007)

(2) Characterization of a putative phosphorylation switch: adaptation of SPOT synthesis to analyze PDZ domain regulation mechanisms.
Boisguerin et al. Chembiochem (2007)




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