APC/C degron repository

APC/C degron instances

Figure 1. Structure of the APC/C
Structure of the H. sapiens APC/C holoenzyme (PDB:4UI9). Structure shows the approximate binding regions of the IR and LRRL tails. The protein coloured in red is the activator. For full details of binding sites see Chang et al.

Gene	Protein	Hit	Type	Start	End	Species	Reference
GIG1	Protein GIGAS CELL1	rtlmtMR	IR tail	242	243	A. thaliana	¹
KIF18A	Kinesin-like protein KIF18A	iskgnLR	IR tail	897	898	H. sapiens	²
NEK2	Serine/threonine-protein kinase Nek2	rqilgMR	IR tail	444	445	H. sapiens	³
PYM	Protein POLYCHOME	rtlmsMR	IR tail	258	259	A. thaliana	⁴
FBXO5	F-box only protein 5	gtkksKKNLRRL	LRRL tail	441	447	H. sapiens	^5,6
E2F1	Transcription factor E2F1	-	Region	358	437	H. sapiens	⁷
EHMT1	Histone-lysine N-methyltransferase EHMT1	-	Region	426	922	H. sapiens	⁸
EHMT2	Histone-lysine N-methyltransferase EHMT2	-	Region	772	1296	H. sapiens	⁸
FOB1	DNA replication fork-blocking protein FOB1	vntfaLANEADNscnnn	Region	417	423	S. cerevisiae	⁹
IQG1	Ras GTPase-activating-like protein IQG1	-	Region	1	42	S. cerevisiae	¹⁰
mtrm	Protein matrimony	nrtptLKERRWNtlkvn	Region	21	27	D. melanogaster	¹¹
NEK2	Serine/threonine-protein kinase Nek2	-	Region	420	445	H. sapiens	³
AURKB	Aurora kinase B	lstlpQRVLrkepv	Region	26	29	H. sapiens	¹²
AURKA	Aurora kinase A	nsgqaQRVLcpsns	Region	45	48	H. sapiens	¹³
CDC20	Cell division cycle protein 20 homolog	ssrktCRYIPSLpdril	Region	165	171	H. sapiens	^14,15

References

(1) "OSD1 promotes meiotic progression via APC/C inhibition and forms a regulatory network with TDM and CYCA1;2/TAM." Cromer L, Heyman J, Touati S, Harashima H, Araou E, Girard C, Horlow C, Wassmann K, Schnittger A, De Veylder L, Mercier R. PLoS Genet.. 2012 ;8 (7):e1002865

(2) "Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex." Sedgwick GG, Hayward DG, Di Fiore B, Pardo M, Yu L, Pines J, Nilsson J. EMBO J.. 2013 Jan;32 (2):303-14

(3) "APC/C-mediated destruction of the centrosomal kinase Nek2A occurs in early mitosis and depends upon a cyclin A-type D-box." Hames RS, Wattam SL, Yamano H, Bacchieri R, Fry AM. EMBO J.. 2001 Dec;20 (24):7117-27

(4) "Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome." Heyman J, Van den Daele H, De Wit K, Boudolf V, Berckmans B, Verkest A, Alvim Kamei CL, De Jaeger G, Koncz C, De Veylder L. Plant Cell. 2011 Dec;23 (12):4394-410

(5) "Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex." Wang W, Kirschner MW. Nat. Cell Biol.. 2013 Jul;15 (7):797-806

(6) "Electron microscopy structure of human APC/C(CDH1)-EMI1 reveals multimodal mechanism of E3 ligase shutdown." Frye JJ, Brown NG, Petzold G, Watson ER, Grace CR, Nourse A, Jarvis MA, Kriwacki RW, Peters JM, Stark H, Schulman BA. Nat. Struct. Mol. Biol.. 2013 Jul;20 (7):827-35

(7) "Regulation of E2F1 by APC/C Cdh1 via K11 linkage-specific ubiquitin chain formation." Budhavarapu VN, White ED, Mahanic CS, Chen L, Lin FT, Lin WC. Cell Cycle. 2012 May;11 (10):2030-8

(8) "DNA damage signaling triggers degradation of histone methyltransferases through APC/C(Cdh1) in senescent cells." Takahashi A, Imai Y, Yamakoshi K, Kuninaka S, Ohtani N, Yoshimoto S, Hori S, Tachibana M, Anderton E, Takeuchi T, Shinkai Y, Peters G, Saya H, Hara E. Mol. Cell. 2012 Jan;45 (1):123-31

(9) "The anaphase promoting complex regulates yeast lifespan and rDNA stability by targeting Fob1 for degradation." Menzel J, Malo ME, Chan C, Prusinkiewicz M, Arnason TG, Harkness TA. Genetics. 2014 Mar;196 (3):693-709

(10) "Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis." Ko N, Nishihama R, Tully GH, Ostapenko D, Solomon MJ, Morgan DO, Pringle JR. Mol. Biol. Cell. 2007 Dec;18 (12):5139-53

(11) "A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony." Whitfield ZJ, Chisholm J, Hawley RS, Orr-Weaver TL. PLoS Biol.. 2013 Sep;11 (9):e1001648

(12) "Mechanism of Aurora-B degradation and its dependency on intact KEN and A-boxes: identification of an aneuploidy-promoting property." Nguyen HG, Chinnappan D, Urano T, Ravid K. Mol. Cell. Biol.. 2005 Jun;25 (12):4977-92

(13) "Requirements for the destruction of human Aurora-A." Crane R, Kloepfer A, Ruderman JV. J. Cell. Sci.. 2004 Dec;117 (Pt 25):5975-83

(14) "The CRY box: a second APCcdh1-dependent degron in mammalian cdc20." Reis A, Levasseur M, Chang HY, Elliott DJ, Jones KT. EMBO Rep.. 2006 Oct;7 (10):1040-5

(15) "Molecular basis of APC/C regulation by the spindle assembly checkpoint." Alfieri C, Chang L, Zhang Z, Yang J, Maslen S, Skehel M, Barford D. Nature. 2016 Aug; ():