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APC/C degron instances


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Figure 1. Sequence logo of the D box degron
A relative frequency sequence logo constructed by subtracting the expected frequency of an amino acid from the observed frequency of an amino acid. A positive score suggests that a residues is enriched , a negative score sugests that a residue is depleted, and score of zero suggest that a residue is occurring at the expected frequency. The logo was constructed from all experimentally characterised D box degrons.


Gene Protein Hit Type Start End Species Reference
ACM1APC/C-CDH1 modulator 1lreggRIALKDLsvdefD box119125S. cerevisiae1,2,3,4
ACM1APC/C-CDH1 modulator 1spskkRTILSSKninqkD box814S. cerevisiae1,2,3,4
Alk1Serine/threonine-protein kinase Haspin homolog ALK1senrlRTPLKPLvnhskD box224230S. cerevisiae5
Alk2Serine/threonine-protein kinase Haspin homolog ALK2tnskfRTPLRPIsnqstD box150156S. cerevisiae5
AMA1Meiosis-specific APC/C activator protein AMA1gtkfgRILLYDQkeffhD box296302S. cerevisiae6
ANLNActin-binding protein anillinhakraRQPLSEAsnqqpD box4147H. sapiens7
ANLNActin-binding protein anillinrtrarRENLQRKmaerpD box1622H. sapiens7
ASE1Anaphase spindle elongation proteinhavkpRQLFPIPlnkvdD box760766S. cerevisiae8
aspProtein abnormal spindleahpppQAPLVEKnvyktD box202208D. melanogaster9
AT3G14190PATRONUS kpggaRKALNDItnksgD box4652A. thaliana10
AURKBAurora kinase BhnpseRLPLAQVsahpwD box315321H. sapiens11
BARD1BRCA1-associated RING domain protein 1lcsklRNLLHDNelsdlD box112118H. sapiens12
BARD1BRCA1-associated RING domain protein 1awahsRAALDRLekllrD box3844H. sapiens12
BCL2L11Bcl-2-like protein 11fifmrRSSLLSRsssgyD box8591H. sapiens13
BCL2L11Bcl-2-like protein 11gpfatRSPLFIFmrrssD box7682H. sapiens13
BUB1BMitotic checkpoint serine/threonine-protein kinase BUB1 betassvpqRSTLAELkskgkD box224230H. sapiens14,15
ccna1Cyclin-A1hakraRQPLSEAsnqqpD box4147X. laevis16,17
CCNA2Cyclin-A2qqprtRAALAVLksgnpD box4753H. sapiens18
CCNB1G2/mitotic-specific cyclin-B1pglrpRTALGDIgnkvsD box4248H. sapiens19,20,21,22
ccnb1G2/mitotic-specific cyclin-B1pglrpRTALGDIgnkaeD box3642X. laevis16,17,20
CCNB3G2/mitotic-specific cyclin-B3galkkRSAFEDLtnasqD box6066H. sapiens23
cdc13G2/mitotic-specific cyclin cdc13nvpkkRHALDDVsnfhnD box5965S. pombe24
cdc13G2/mitotic-specific cyclin cdc13nvpkkRHALDDVsnfhnD box5965S. pombe16,17
CDC20APC/C activator protein CDC2aisgnRSVLSIAsptklD box1723S. cerevisiae25
CDC5Cell cycle serine/threonine-protein kinase CDC5/MSD2aqkkkREKLSALcktppD box6167S. cerevisiae26,27
CDC5Cell cycle serine/threonine-protein kinase CDC5/MSD2kqlntRSKLVHTpikgnD box1723S. cerevisiae26,27
CDC6Cell division control protein 6 homologlplspRKRLGDDnlcntD box5662H. sapiens28
CDCA3Cell division cycle-associated protein 3skvlgRSPLTILqddnsD box198204H. sapiens29
CDKN1ACyclin-dependent kinase inhibitor 1gprrgRDELGGGrrpgtD box8692H. sapiens30,31
cig2G2/mitotic-specific cyclin cig2inlprRTVLSDVsnvgkD box5157S. pombe32
CIN8Kinesin-like protein CIN8pnlskKMPLRLSninsnD box954960S. cerevisiae33,4
CLB2G2/mitotic-specific cyclin-2lrnvqRLALNNVtnttfD box2531S. cerevisiae34
CLB5S-phase entry cyclin-5qdskpRRALTDVpvnnnD box5662S. cerevisiae35
cut2SecurinsvtvpRTVLGGKstnisD box5258S. pombe36
cut2SecuringagskRAPLGSTkqsnaD box3339S. pombe36
CycAG2/mitotic-specific cyclin-AnalapRANFAVLngnnnD box4652D. melanogaster37
CYCA2-3Cyclin-A2-3nkrkkRAVLGEItnvnsD box5561A. thaliana38
CYCA3-1Putative cyclin-A3-1rinkkRVVLGELpnlsnD box3541A. thaliana39
CycBG2/mitotic-specific cyclin-BeattkRAALGDLqnrgiD box3743D. melanogaster40
cycB1Cyclin B1adgrnRRALGDIgnlvpD box3137N. tabacum39,41
CycB3G2/mitotic-specific cyclin-B3ndkikRSALGNLtnnvkD box7581D. melanogaster40
DBF4DDK kinase regulatory subunit DBF4krsleRLELQQQqhlheD box6268S. cerevisiae42,43
DBF4DDK kinase regulatory subunit DBF4tkmiiRSPLKETdtnlkD box1016S. cerevisiae43
DLGAP5Disks large-associated protein 5tkiahRKSLSQKenrhkD box2632H. sapiens12
DNM1LDynamin-1-like proteinvtcllRKRLPVTnemvhD box473479H. sapiens44
DVL1L1Putative Segment polarity protein dishevelled homolog DVL-1-likeleirdRMWLKITianavD box409415H. sapiens45
E1Replication protein E1sdsnaRAFLATNsqakhD box346352Bovine papillomavirus type 146
egg-3Protein EGG-3magwlRDRLVPSmsdcsD box130136C. elegans47
egg-3Protein EGG-3cdeetRILLVEKlmnakD box96102C. elegans47
ERBB4Receptor tyrosine-protein kinase erbB-4iqgddRMKLPSPndskfD box992998H. sapiens48
ETS2Protein C-ets-2vansyRGTLKRQpafdtD box2026H. sapiens49
FAM64AProtein FAM64ArqfqrRLPLRAVnlragD box5359H. sapiens50
FAN1Fanconi-associated nuclease 1dkkrpRRSLSISknkkkD box1420H. sapiens51
FBXO5F-box only protein 5eyvmfRTPLASVqksaaD box322328H. sapiens52
FBXO5F-box only protein 5eyvmfRTPLASVqksaaD box322328H. sapiens22
FBXW5F-box/WD repeat-containing protein 5akeglRHFLDRVlegraD box303309H. sapiens53
FIN1Filament protein FIN1snksnRRSLRDIgntigD box713S. cerevisiae54
FOXM1Forkhead box protein M1ilkrrRLPLPVQnapseD box1521H. sapiens55
FOXM1Forkhead box protein M1plilkRRRLPLPvqnapD box1319H. sapiens55
FOXM1Forkhead box protein M1mktspRRPLILKrrrlpD box612H. sapiens55
FOXRED2FAD-dependent oxidoreductase domain-containing protein 2hilplRRFLENCldtdlD box582588H. sapiens56
FOXRED2FAD-dependent oxidoreductase domain-containing protein 2mlsrsRVRLSWAthyvgD box246252H. sapiens56
GAS2L3GAS2-like protein 3lkspgRTPLSIVslpqsD box610616H. sapiens57
GIG1Protein GIGAS CELL1pswypRTPLRDIthivrD box104110A. thaliana58
GMNNGemininnssvpRRTLKMIqpsasD box2329H. sapiens59
GRIA1Glutamate receptor 1fvlqlRPELQDAlisiiD box126132H. sapiens60
GRIA1Glutamate receptor 1ehaafRFALSQLteppkD box4349H. sapiens60
HECW2E3 ubiquitin-protein ligase HECW2evwqrRGSLEGAaaaaeD box740746H. sapiens61
HMMRHyaluronan mediated motility receptorenfalKTPLKEGntncyD box702708H. sapiens12
HMMRHyaluronan mediated motility receptorevsklRCQLAKKkqsetD box659665H. sapiens12
HOXC10Homeobox protein Hox-C10wfqnrRMKLKKMnrenrD box320326H. sapiens62
HOXC10Homeobox protein Hox-C10apfeqRASLNPRaehleD box177183H. sapiens62
HSL1Probable serine/threonine-protein kinase HSL1eqkpkRAALSDItnsfnD box828834S. cerevisiae22,63
ID1DNA-binding protein inhibitor ID-1rglpvRAPLSTLngeisD box126132H. sapiens64
ID2DNA-binding protein inhibitor ID-2qnqasRTPLTTLntdisD box100106H. sapiens64
InvsInversinqrersRKELFRRknkaaD box907913M. musculus65
InvsInversinqdkegRTALHWScnngyD box490496M. musculus65
KIF11Kinesin-like protein KIF11lvtksRLPLRAQinl--D box10471053H. sapiens66
KIFC1Kinesin-like protein KIFC1-mdpqRSPLLEVkgnieD box511H. sapiens67
MAD3Spindle assembly checkpoint component MAD3rpkisRKALVSKsltpsD box429435S. cerevisiae68
MCL1Induced myeloid leukemia cell differentiation protein Mcl-1sgatsRKALETLrrvgdD box207213H. sapiens69
MCPH1Microcephalin, isoform BaasstRRPLHDSnlgdfD box3642D. melanogaster70
mes1Protein mes1qrplqRRPLQELsielvD box3440S. pombe71
MOC1Protein MONOCULM 1aapstRDLLLACadllqD box5157O. sativa72
Myf5Myogenic factor 5atmreRRRLKKVnqafeD box9399M. musculus46
NEK2Serine/threonine-protein kinase Nek2sllkeRKFLSLAsnpelD box361367H. sapiens73
NEUROD2Neurogenic differentiation factor 2lqlnsRNFLTEQgadgaD box213219H. sapiens74
NINLNinein-like proteinhyqdlRTQLETKvnyyeD box633639H. sapiens75
NRM1Transcription factor NRM1simkqRLPLGEFssskiD box713S. cerevisiae76
NUSAP1Nucleolar and spindle-associated protein 1kvlgmRRGLILAed---D box433439H. sapiens12
Orc1Origin recognition complex subunit 1anylpASPLTEKnakveD box292298D. melanogaster4,77,9
PDS1SecurinaqqqgRLPLAAKdnnrsD box8591S. cerevisiae4,78
pimPimples proteintnsvlKKPLGNLdnvmhD box3036D. melanogaster79
Piwil1Piwi-like protein 1yniifRRLLKIMnlqqiD box218224M. musculus80
PLK1Serine/threonine-protein kinase PLK1ldpsnRKPLTVLnkgleD box337343H. sapiens81
PTTG1SecurinlpkatRKALGTVnrateD box6167H. sapiens21,82,83
PYMProtein POLYCHOMEpswypRTPLRDIsavvrD box119125A. thaliana84
RAA1Flowering-promoting factor 1-like protein 1fqfhkRGSLDLislpadD box7381O. sativa85
RSS1RSS1 proteinppvstRKPLQDVsntakD box6369O. sativa86
SGO1ShugoshinnspkkSSPLLDItnkseD box486492S. cerevisiae87
SGOL1Shugoshin-like 1tqqspHLSLKDItnvslD box438444H. sapiens88
Sgol1Shugoshin-like 1tqespRCSLKDVtnilqD box428434M. musculus88
SJAG_031983-oxoacyl-[acyl-carrier-protein] reductase Oar2mqcilRTNLEAAielsqD box106112S. japonicus89
SJAG_031983-oxoacyl-[acyl-carrier-protein] reductase Oar2iqelvRQNLPTLdaavhD box7278S. japonicus89
SKILSki-like proteinvggekRLCLPQVlnsvlD box164170H. sapiens90
SKP2S-phase kinase-associated protein 2---mhRKHLQEIpdlssD box39H. sapiens91,92
SP100Nuclear autoantigen Sp-100eereeRSGLQLSleqgtD box165171H. sapiens93
spo13Meiosis-specific protein SPO13skrkvQKPLQEKtpnlrD box2329S. cerevisiae4,94
TEX14Inactive serine/threonine-protein kinase TEX14sprvqRYGLHPDvnvylD box533539H. sapiens95
TOS4Protein TOS4iselsRDELRSIlsdakD box414420S. cerevisiae96
TRIB3Tribbles homolog 3fadreRKKLVLEnledsD box195201H. sapiens97
TTKDual specificity protein kinase TTKaeigyRNSLRQTnktkqD box256262H. sapiens98,99
Ube2cUbiquitin-conjugating enzyme E2 CalydvRTILLSIqsllgD box129135M. musculus100
UIMC1BRCA1-A complex subunit RAP80sgdtsRHCLPTLadakgD box255261H. sapiens101
UME6Transcriptional regulatory protein UME6hnktsRATLMNSsqdgkD box4046S. cerevisiae102
YHP1Homeobox protein YHP1fnayeRKPLGEVdlnsfD box340346S. cerevisiae76
YHP1Homeobox protein YHP1kgkenRLKFNAYerkplD box332338S. cerevisiae76
ZC3HC1Nuclear-interacting partner of ALKlrkakRARLCSSsssdtD box402408H. sapiens103
ZC3HC1Nuclear-interacting partner of ALKegrpeRLPLVPEsprrmD box314320H. sapiens103


References


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      (40) "Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3." Sigrist S, Jacobs H, Stratmann R, Lehner CF. EMBO J.. 1995 Oct;14 (19):4827-38

      (41) "Expression of a nondegradable cyclin B1 affects plant development and leads to endomitosis by inhibiting the formation of a phragmoplast." Weingartner M, Criqui MC, Mészáros T, Binarova P, Schmit AC, Helfer A, Derevier A, Erhardt M, Bögre L, Genschik P. Plant Cell. 2004 Mar;16 (3):643-57

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      (44) "Regulation of mitochondrial morphology by APC/CCdh1-mediated control of Drp1 stability." Horn SR, Thomenius MJ, Johnson ES, Freel CD, Wu JQ, Coloff JL, Yang CS, Tang W, An J, Ilkayeva OR, Rathmell JC, Newgard CB, Kornbluth S. Mol. Biol. Cell. 2011 Apr;22 (8):1207-16

      (45) "Regulation of ciliary polarity by the APC/C." Ganner A, Lienkamp S, Schäfer T, Romaker D, Wegierski T, Park TJ, Spreitzer S, Simons M, Gloy J, Kim E, Wallingford JB, Walz G. Proc. Natl. Acad. Sci. U.S.A.. 2009 Oct;106 (42):17799-804

      (46) "Constitutive instability of muscle regulatory factor Myf5 is distinct from its mitosis-specific disappearance, which requires a D-box-like motif overlapping the basic domain." Lindon C, Albagli O, Domeyne P, Montarras D, Pinset C. Mol. Cell. Biol.. 2000 Dec;20 (23):8923-32

      (47) "Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the oocyte-to-zygote transition." Stitzel ML, Cheng KC, Seydoux G. Curr. Biol.. 2007 Sep;17 (18):1545-54

      (48) "HER4 D-box sequences regulate mitotic progression and degradation of the nuclear HER4 cleavage product s80HER4." Strunk KE, Husted C, Miraglia LC, Sandahl M, Rearick WA, Hunter DM, Earp HS, Muraoka-Cook RS. Cancer Res.. 2007 Jul;67 (14):6582-90

      (49) "The adaptor protein of the anaphase promoting complex Cdh1 is essential in maintaining replicative lifespan and in learning and memory." Li M, Shin YH, Hou L, Huang X, Wei Z, Klann E, Zhang P. Nat. Cell Biol.. 2008 Sep;10 (9):1083-9

      (50) "RCS1, a substrate of APC/C, controls the metaphase to anaphase transition." Zhao WM, Coppinger JA, Seki A, Cheng XL, Yates JR, Fang G. Proc. Natl. Acad. Sci. U.S.A.. 2008 Sep;105 (36):13415-20

      (51) "Human KIAA1018/FAN1 nuclease is a new mitotic substrate of APC/C(Cdh1)." Lai F, Hu K, Wu Y, Tang J, Sang Y, Cao J, Kang T. Chin J Cancer. 2012 Sep;31 (9):440-8

      (52) "Emi1 stably binds and inhibits the anaphase-promoting complex/cyclosome as a pseudosubstrate inhibitor." Miller JJ, Summers MK, Hansen DV, Nachury MV, Lehman NL, Loktev A, Jackson PK. Genes Dev.. 2006 Sep;20 (17):2410-20

      (53) "The SCF-FBXW5 E3-ubiquitin ligase is regulated by PLK4 and targets HsSAS-6 to control centrosome duplication." Puklowski A, Homsi Y, Keller D, May M, Chauhan S, Kossatz U, Grünwald V, Kubicka S, Pich A, Manns MP, Hoffmann I, Gönczy P, Malek NP. Nat. Cell Biol.. 2011 Aug;13 (8):1004-9

      (54) "Cdk and APC activities limit the spindle-stabilizing function of Fin1 to anaphase." Woodbury EL, Morgan DO. Nat. Cell Biol.. 2007 Jan;9 (1):106-12

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